Hasan Moradian; Ali Esmailizadeh; Mohammadreza Mohammadabadi
Volume 15, Issue 2 , October 2013, , Pages 89-99
Abstract
The purpose of this study was to identify genomic regions of quantitative trait loci (QTL), affectingcarcass traits on chromosome 1 in an F2 population of Japanese quail. For this purpose, a three-generationresource population was developed by using two distinct Japanese quail strains, wild (meat type) ...
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The purpose of this study was to identify genomic regions of quantitative trait loci (QTL), affectingcarcass traits on chromosome 1 in an F2 population of Japanese quail. For this purpose, a three-generationresource population was developed by using two distinct Japanese quail strains, wild (meat type) andwhite (layer type). Eight pairs of white and wild birds were crossed reciprocally and 34 F1 birds wereproduced. The F1 birds were intercrossed to generate 422 F2 offspring. Phenotypic data including weightof carcass parts were collected on F2 birds. All of the animals from three generations (472 birds) weregenotyped for eight microsatellite markers on chromosome 1. QTL analysis was performed with leastsquares interval mapping method fitting three various statistical models. Significant QTL were identifiedfor breast weight, carcass weight, head weight and percentage of breast. There was also evidence forimprinted QTL affecting breast weight, a carcass part of high economic value, on chromosome 1. Theproportion of the F2 phenotypic variation explained by the significant additive, dominance and imprintedQTL effects ranged from 1.8 to 2.3, 1.2 to 3.3 and 0.5 to 2.2 percent, respectively.
Mohammad Reza Mohammad abadi; Akram Mohammadi
Volume 12, Issue 2 , October 2010, , Pages 61-67
Abstract
Beta-lactoglobulin (LGB) is one of the proteins in the mammals’ milk synthesized by the epithelial cells of the mammary glands and affects the quality and coagulation of the milk. The aim of this study was to determine allele frequencies in LGB gene of Native and Holstein cattle in Kerman province ...
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Beta-lactoglobulin (LGB) is one of the proteins in the mammals’ milk synthesized by the epithelial cells of the mammary glands and affects the quality and coagulation of the milk. The aim of this study was to determine allele frequencies in LGB gene of Native and Holstein cattle in Kerman province using PCR-RFLP method. Genetic polymorphism, for 100 DNA samples in the locus was determined by digestion of PCR products with endonuclease HaeIII (LGB), followed by electrophoresis in agarose gel stained with ethidium bromide. Allele frequency for the A and B alleles in Holstein cattle was 0.62 and 0.38 and in Native cattle was 0.55 and 0.45, respectively. Average heterozygosity in Holstein and Native cattle was 0.47 and 0.46, respectively. Results indicated that the studied populations were not in Hardy-Weinberg equilibrium for the LGB locus.